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Growth characteristics of an ainS mutant of V. fischeri. Growth in SWT medium of wild-type (circles) and ainS (squares) cells was monitored. At the indicated time points, samples were removed and the OD600 (A) and pH (B) were measured, and an aliquot was diluted and plated to determine CFU/ml (C). The pH of an uninoculated flask remained between 7.3 and 7.5. The average results of two separate experiments was plotted.
