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Effect of quorum-sensing signals on the expression of acs by V. fischeri. The relative level of transcription of an acs′-lacZ+ promoter reporter was determined with strains of wild-type and quorum-sensing mutants containing the acs fusion plasmid. The SWT culture medium was supplemented with either 40 nM 3OC6-HSL (white bars), 40 nM C8-HSL (striped bars) or a buffer blank (black bars). The standard errors for three replicate experiments are shown. B-gal, β-galactosidase.
