FIG. 2.

RrgA and RrgC form a complex in the cell wall compartment. The bacterial cell wall (cw) fractions of the wild type; rrgA, rrgB, and rrgC mutants; and an rrgB rrgC double mutant were separated by SDS-polyacrylamide gel electrophoresis, transferred to nitrocellulose, and probed with anti-RrgA (A) or anti-RrgC (B) antiserum. The band representing an RrgA/RrgC multimer is indicated with an asterisk, and the large and small RrgA bands are represented by one or two crosses, respectively, with a key below the panel. The values on the left of each panel are molecular weight marker sizes in kilodaltons.