FIG. 2.

Time dependency of ATP and ADP uptake into E. coli cells expressing the ATP/ADP translocase NTT1_Li from L. intracellularis. Shown is the time-dependent uptake of [α-³²P]ATP (A) and [α-³²P]ADP (B) mediated by E. coli expressing NTT1_Li (black diamonds) or into noninduced cells harboring pLiNTT1 (control, gray squares). Cells were incubated in phosphate buffer medium containing 50 μM labeled nucleotide for the indicated time periods. Data are the mean of at least three independent experiments. Standard errors are given.