FIG. 3.
Alteration of the ggpPS-specific mRNA steady-state levels in S. rhizophila DSM 14405 after a salt shock of 3% NaCl was applied (A). During the complete growth phase, samples were taken at the given time points, and total RNA was isolated and analyzed by hybridization with a 32P-labeled probe. The signals were quantified using a phosphorimager and were related to a hybridization signal of a 16S-rRNA gene probe applied to the same filters. The mRNA levels obtained at time point 0 were set to the level 10, while all other mRNA levels represent induction ratios relative to this time point. For comparison, the amount of GG in cells (panel A, black columns) and in the medium (panel A, gray columns) was estimated. (B) The growth curve is displayed for S. rhizophila cells shocked with 3% NaCl at time point 0 h.