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. 2008 Jun 27;190(17):5898–5906. doi: 10.1128/JB.00643-08

FIG. 5.

FIG. 5.

Complementation of the defined Synechocystis sp. strain PCC 6803 mutants defective in GG synthesis by the ggpPS gene from S. rhizophila. The salt resistance and accumulation of GG were analyzed in cells of the Synechocystis wild type (WT), the salt-sensitive mutants of Synechocystis defective in GgpS (ΔggpS) or GgpP (ΔstpA), and the mutant strains expressing the Synechocystis ggpS gene (ΔggpS + SynggpS), the full-length ggpPS gene from S. rhizophila (ΔggpS + StenoggpPS and ΔstpA + StenoggpPS, respectively), the truncated version of the ggpPS gene from S. rhizophila (ΔggpS + StenoggpS), and the gfp gene (ΔggpS + gfp), respectively. (A) Effect of NaCl on the viability of cells grown for 10 days on BG11 medium supplemented with 0 or 2% NaCl (pH 8). (B) GG accumulation in cells of the strains grown in liquid BG11 medium supplemented with 0 or 2% NaCl for 1 day.