TABLE 1.

Oligonucleotides used for amplification and cloning of the corresponding genes

Primer	Sequencea	Restriction site	Application
ggpS univ 5A	AARGAAGCCTTYTGGCC		Cloning of internal ggpPS fragments
ggpS univ 3A	GTGTGGTGGAARAAGGC
AvXcPa AS 63	ACCGGCCGCAGCCTGGAA
AvXcPa AS 111	CGCCGCTGCTCCTAYTTCTGC
AvXcPa AS 139	CGCCGCTGCTCCTAYTTCTGC
ggpSD5o	TGCGGTTGACCTTCAGGA
ggpS3i	TTTCAGGTCCGGGCGCAGTT		Inverse PCR of 3′ end of ggpPS
tps univ 5A	TTTTTTYTGCACATYCC		Cloning of the partial tps gene
tps univ 3A	ATGAACYTGGTGGCCAA
ggp5C	TTYCTGCACACCCCSTTYCC
ggpS5i	ATGATGCATGTGTTCTGGGA		Labeling of the ggpPS probe
GgpS5′Xho	CTCGAGTTGACTGTATCGACA	XhoI	Cloning of the complete ggpPS gene into expression vector
ggpS3′Hind	AAGCTTTCAACCGCGCACCGC	HindIII
ggpS5′AS284	CTCGAGGCCGGCGCTGACCTG	XhoI	Cloning of the truncated ggpPS gene
Syn_compl.5′	GGGTACCATGAATTCATCCCTTGTGAT	KpnI	Amplification of ggpS from Synechocystis sp. strain PCC 6803
Syn_compl.3′	GTCTAGACTACATTTGGGGGGGCTCTC	XbaI
SF_compl.5′	GGGTACCATGACTGTATCGACACCCTCT	KpnI	Amplification of full-length ggpPS and its truncated version from S. rhizophila for complementation
Sk_compl.5′	GGGTACCATGGGCGCTGACCTGGTGATG	KpnI
SF_compl.3′	GGAGCTCTCAACCGCGCACCGCCTCCA	SacI
proggpS-5′	GGGGTACCTCCAGGCATATTAGTTCA	KpnI	Amplification of the ggpS (strain PCC 6803) promoter region from nucleotide −193 to +495 (20)
proggpS-3′ (1027-KpnI)	GGGTACCATTCATAAAATCAGCGG	KpnI

^aAdded restriction sites are underlined.