TABLE 1.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Description | Reference or source |
|---|---|---|
| Streptococcus pneumoniae strains | ||
| R6 | Penicillin-susceptible laboratory strain derived from R36A | RUa collection |
| R6Cho− | Choline-independent derivative of R6 | 19 |
| R6Chi | Spontaneous mutant of S. pneumoniae strain R6 carrying a G→T point mutation at nucleotide position 700 in spr1150 | 4 |
| R6Cho−spr1150ID | Insertion/duplication mutant of R6Cho−, with inactivated spr1150 | This study |
| R6Cho−ΔlicD1D2 | licD1-licD2 deletion mutant of R6Cho− | This study |
| R6Cho−Δlic2 | The lic2 operon deletion mutant of R6Cho− | This study |
| D39 | Type 2 capsular virulent S. pneumoniae strain | RU collection |
| D39Cho− | Choline-independent derivative of D39 | 12 |
| D39Cho−ΔlicD1D2 | licD1-licD2 deletion mutant of D39Cho− | This study |
| D39Cho−Δlic2 | The lic2 operon deletion mutant of D39Cho− | This study |
| D39Chi | Choline-independent derivative of D39 carrying G700T point mutation in the tacF gene | 4 |
| D39ChiΔlicD1D2 | licD1-licD2 deletion mutant of D39Chi | This study |
| Plasmids | ||
| pJDC9 | E. coli plasmid; Ermr | 3 |
| pR410 | E. coli plasmid; Kanr | 21 |
| pGEM3Z | E. coli plasmid; Ampr | 29 |
| pSPR1150ID | Plasmid pJDC9 carrying an internal DNA fragment of tacF amplified with spr1150IDF-RI and spr1150IDR-BamHI primers | This study |
| pΔD1D2DU3 | Plasmid pGEM3Z carrying an insert composed of upstream flank of the licD1 gene and downstream flank of the licD2 gene | This study |
| pΔD1D2DU31 | Plasmid pGEM3Z carrying an insert composed of upstream flank of the licD1 gene/kanamycin resistance cassette and downstream flank of the licD2 gene | This study |
| pΔLIC2 | Plasmid pGEM3Z carrying an insert composed of upstream flank of the spr1150 (tacF) gene/kanamycin resistance cassette and downstream flank of the licD2 gene | This study |
a
RU, Rockefeller University.