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. 2008 Jun 20;190(17):5890–5897. doi: 10.1128/JB.00459-08

FIG. 3.

FIG. 3.

Competition in DNA binding between NemR and RutR. Fluorescence-labeled ydhL-nemR spacer DNA probe (0.5 pmol; Fig. 2) was incubated at 37°C for 30 min in the absence (lane 1) or presence of increasing concentrations of NemR (lane 2, 0.5 pmol; lane 3, 1.0 pmol; lane 4, 2.0 pmol) or RutR (lane 5, 1.0 pmol: lane 6, 2.0 pmol; lane 7, 3.0 pmol). In the reactions shown in lanes 8 to 13, the increasing amounts of NemR (lane 8, 0.5 pmol; lane 9, 1.0 pmol; lane 10, 2.0 pmol) were added in the presence of a fixed amount of RutR (2.0 pmol) or the increasing amounts of RutR (lane 11, 1.0 pmol; lane 12, 2.0 pmol; lane 13, 3.0 pmol) were added in the presence of a fixed amount of NemR (2.0 pmol). The reaction mixtures were directly subjected to PAGE.