FIG. 7.

Effect of NEM on DNA-binding activity of NemR. Fluorescence-labeled ydhL-nemR spacer DNA probe (0.2 pmol; Fig. 2 and 6) was incubated at 37°C for 30 min in the absence (lane 1) or presence of either NemR (lane 3, 1.0 pmol) or RutR (lane 2, 1.0 pmol). In the reaction shown in lane 4, fixed amounts of both NemR and RutR were added. In addition, increasing concentrations of NEM were added (lane 5, 0.1 nmol; lane 6, 1.0 nmol; lane 7, 10 nmol). The reaction mixtures were directly subjected to PAGE.