TABLE 1.
Strains and plasmids used in this study
| Strain or plasmid | Characteristicsa | Source or reference |
|---|---|---|
| Strains | ||
| Vibrio fischeri | ||
| ES114 | Wild-type isolate from E. scolopes light organ tissue | 7 |
| AKD800 | ES114 ΔtorECA (allele exchanged from pAKD800) | This study |
| AKD801 | ES114 ΔtorYZ (allele exchanged from pAKD801) | This study |
| AKD802 | ES114 ΔdmsABC (allele exchanged from pAKD802) | This study |
| AKD803 | ES114 ΔtorECA ΔtorYZ | This study |
| AKD804 | ES114 ΔtorECA ΔdmsABC | This study |
| AKD805 | ES114 ΔtorYZ ΔdmsABC | This study |
| AKD806 | ES114 ΔtorECA ΔtorYZ ΔdmsABC | This study |
| AKD807 | ES114 Tn7 PtorYZ-lacZ insertion | This study |
| AKD808 | ES114 Tn7 PtorECA-lacZ insertion | This study |
| AKD809 | ES114 Tn7 PdmsABC-lacZ insertion | This study |
| JB1 | ES114 Δfnr | J. L. Bose |
| KV1585 | ES114 torR::pKV174 | 18 |
| Escherichia coli | ||
| DH5α | F′/endA1 hsdR17 glnV44 thi-1 recA1 gyrA relA1 Δ(lacIZYA-argF)U169 deoR [φ80dlacIΔ(lacZ)M15] | 17 |
| DH5α λpir | λpir derivative of DH5α | 13 |
| CC118 λpir | Δ(ara-leu) araD ΔlacX74 galE galK phoA20 thi-1 rpsE rpoB argE(Am) recA1, lysogenized with λpir | 33 |
| Plasmids | ||
| pVSV105 | E. coli-V. fischeri shuttle vector, RP4 oriT, pES213 and R6K replication origins, Cmr | 14 |
| pVSV209 | E. coli-V. fischeri shuttle vector, RP4 oriT, pES213 and R6K replication origins, promoterless gfp and Cmr, constitutive rfp, Knr | 14 |
| pVSV209torECA | pVSV209 with the torECA promoter region | This study |
| pVSV209torYZ | pVSV209 with the torYZ promoter region | This study |
| pVSV209dmsABC | pVSV209 with the dmsABC promoter region | This study |
| pAKD701 | gfp and Cmr-containing StuI-SanDI fragment in pVSV209 was replaced with a StuI-SanDI lacZ fragment PCR-amplified from E. coli MG1655 using lacZF and lacZR, rfp removed by NotI digestion followed by self-ligation | This study |
| pAKD701torECA | pAKD701 with the torECA promoter region | This study |
| pAKD701torYZ | pAKD701 with the torYZ promoter region | This study |
| pAKD701dmsABC | pAKD701 with the dmsABC promoter region | This study |
| pAKD800 | ΔtorECA allele, R6K and ColE1 replication origins, RP4 oriT, Emr, Knr | This study |
| pAKD801 | ΔtorYZ allele, R6K and ColE1 replication origins, RP4 oriT, Emr, Knr | This study |
| pAKD802 | ΔdmsABC allele, R6K and ColE1 replication origins, RP4 oriT, Emr, Knr | This study |
| pAKD803 | pVSV105 containing the torECA complementing fragment | This study |
| pEVS104 | Conjugative helper plasmid, R6K replication origin, Knr | 37 |
| pEVS107 | pEVS94S derivative, mini-Tn7, mob, Emr, Knr | 23 |
| pEVS107torYZ | pEVS107 containing PtorYZ-lacZ PCR amplified from pAKD701torYZ | This study |
| pEVS107torECA | pEVS107 containing PtorECA-lacZ PCR amplified from pAKD701torECA | This study |
| pEVS107dmsABC | pEVS107 containing PdmsABC-lacZ PCR amplified from pAKD701dmsABC | This study |
a
Cmr, chloramphenicol resistance; Knr, kanamycin resistance; Emr, erythromycin resistance; rfp, red fluorescent protein gene.