. 2008 Jul 7;190(17):5814–5823. doi: 10.1128/JB.00227-08

TABLE 2.

Activity of TMAO reductase promoter-lacZ fusions

Conditions^a	Promoter activity in Miller units^b
	P_torECA-lacZ		P_torYZ-lacZ		P_dmsABC-lacZ
	Without TMAO	With TMAO	Without TMAO	With TMAO	Without TMAO	With TMAO
Aerobic
LBS
Mid-log phase	120 (±6)^d	360 (±35)	8 (±1)	7 (±1)	2 (±0.1)^c	5 (±1)
Stationary phase	170 (±6.3)^c	390 (±58)	100 (±14)^c	60 (±4)	3 (±0.3)^c	5 (±0.4)
MS with glucose
Mid-log phase	170 (±37)	220 (±39)	6 (±1)	5 (±1)	2 (±0.1)	2 (±0.4)
Stationary phase	340 (±16)	481 (±145)	20 (±5)	10 (±1)	4 (±1)^c	2 (±0.2)
MS without glucose
Mid-log phase	300 (±33)	440 (±73)	17 (±1.1)^c	12 (±1.2)	3 (±0.1)	2 (±0.2)
Stationary phase	810 (±63)	890 (±65)	30 (±1)^d	17 (±0.9)	5 (±0.3)^d	3 (±0.2)
Anaerobic
LBS
Mid-log phase	340 (±1)	500 (±86)	90 (±1)^d	60 (±6)	70 (±6)^d	10 (±1)
Stationary phase	320 (±15)^d	1,220 (±81.9)	100 (±3.5)^d	200 (±13)	70 (±2)^d	40 (±2)
MS with glucose
Mid-log phase	30 (±5)^d	160 (±23)	70 (±9)^d	10 (±2)	76 (±21)^c	6 (±1)
Stationary phase	50 (±3)	604 (±234)	110 (±7.2)^d	70 (±3)	170 (±11)^d	30 (±2)
MS without glucose
Mid-log phase	ND	780 (±29)	ND	120 (±22)	ND	80 (±5)
Stationary phase	ND	3,170 (±164)	ND	280 (±12)	ND	100 (±9)

Culture conditions included growth in LBS (rich medium) or MS, either aerobically or anaerobically, and with or without 40 mM TMAO. OD₅₉₅ measurements were used to determine the mid-log and stationary phases (see Table 3).

Promoter activity is reported for the average of three independent cultures (± standard error of the mean). Significant differences between Miller unit values for a particular promoter with and without TMAO under specific growth conditions were determined using Student's t test. ND, not determined; V. fischeri will not grow under these conditions.

P < 0.05.

P < 0.01.