TABLE 1.
Strains and plasmids used in the study
| Strain or plasmid | Properties | Reference |
|---|---|---|
| Plasmids | ||
| pHSS6 | E. coli cloning vector (Kanr) | 28 |
| pIDN1 | N. gonorrhoeae insertion/duplication plasmid (Ermr) | 15 |
| pIDN3 | N. gonorrhoeae insertion/duplication plasmid (Ermr) | 15 |
| pKC1 | N. gonorrhoeae insertion/duplication, positive/negative selection plasmid (Ermr/Strs) | 3 |
| pKC3 | ltgA in pHSS6 (Kanr) | 3 |
| pKH61 | ltgA deletion plasmid (Kanr) | This work |
| pKH82 | ltgA deletion in pIDN1 (Ermr) | This work |
| pKH84 | pKH82 plus flanking sequence (Ermr) | This work |
| pKH75 | ltgE insertion/duplication plasmid (Ermr) | This work |
| pKC4 | ltgA disruption plasmid (Ermr/Strs/Kanr) | 3 |
| pKC12 | ltgD PCR product digested with EcoRV/BclI cloned into BamHI, EcoRV sites of pKC1 (Ermr/Strs) | This work |
| pKC14 | ltgD internal deletion plasmid, formed by SmaI digestion of pKC12 (Ermr/Strs) | This work |
| pKC16 | ltgD internal deletion plasmid, ltgD region subcloned into HindIII/XbaI sites of pHSS6 (Kanr) | This work |
| pKC18 | ltgD disruption plasmid, ermC/rpsL cloned into BglI site of pKC16 (Kanr/Ermr/Strs) | This work |
| N. gonorrhoeae strains | ||
| MS11 | Wild type (Strr) | 31 |
| KC119 | MS11 ltgD | This work |
| KH560 | MS11 ltgA ltgD | This work |
| KH571 | MS11 ltgE | This work |
| ND500 | MS11 with the GGI deleted | 14 |