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. 2008 Jun 11;82(16):8038–8050. doi: 10.1128/JVI.00721-08

FIG. 3.

FIG. 3.

Downregulation of SC35 and hnRNP H by siRNA alters exon 6D expression. A) HEK-293T cells were transfected with the indicated proviral constructs or pEGFP-N1 as normalizing control and treated with either hnRNP H, SC35, or control siRNAs. mRNA was extracted 72 h after the siRNA treatment and analyzed by qPCR as described for Fig. 2. The product obtained from each reaction was normalized for the content in the pEGFP-N1 gene product and plotted versus the amount expressed by the si-Control-treated cells. Each graph shows the analysis of the viral mRNA species expressed by the indicated proviral clone. B) The graph indicates the quantification by qPCR of the SC35 and hnRNPH mRNA extracted from the respective siRNA-treated cells in comparison with the si-Control treated cells. The right panel shows the amounts of SC35 and hnRNP H proteins present in the siRNA-treated cells in comparison with the si-Control-treated cells.