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Expression and purification of the sapovirus NS6 and NS6-7 proteins and their respective active-site mutants. The cDNA clones pJG-SAP-NS6, pJG-SAP-μNS6, pJG-SAP-NS6-7, and pJG-SAP-μNS6-7 were used for expression in Escherichia coli Rosetta(DE3) cells. The NS6 and NS6-7 proteins as well as their active-site mutants (μNS6 and μNS6-7) were purified by affinity chromatography on a Ni-NTA column followed by gel chromatography. Coomassie staining after SDS-PAGE of eluted fractions is shown.
