FIG. 8.

Inhibition of SeV replication in La-depleted cells. (A) A549 cells were transfected with no siRNA, negative control luciferase siRNA, or La siRNA (all 50 nM). Cells were infected with SeV at 3 multiplicities of infection. Thirty-six hours later, and after another 8 h and 18 h, RNA was harvested, and IFN-β mRNA levels were determined by qRT-PCR. Amounts were expressed as the change over uninfected A549 cells. (B and C) A549 and Vero cells, respectively, were transfected with siRNA and infected with SeV as described above (A), and at 72 h p.i., the viral titer in the extracellular medium was measured by standard plaque assay on Vero cells. Asterisks indicate highly significant changes (P < 0.05).