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Temporal expression of SfMNPV Sf29 and polyhedrin (Sfpolh) genes. RT-PCR analysis of Sf29 and Sfpolh was performed on total RNA extracted from infected larvae at indicated times postinfection. Transcript amplifications were performed using primers Sf29.1 and Sf29.2 for Sf29 and primers Sfpolh.1 and Sfpolh.2 for Sfpolh. The same amount of RNA was used for both Sf29 and Sfpolh amplifications. M, molecular DNA marker of 100 bp Stratagene.
