TABLE 1.
Primers used in this study
| Primer | Sequence | Amplification purpose |
|---|---|---|
| Sf29del.1 | 5′-TGATTTATTTATATTTTATTTACCCGGGTGGACAGCAAGCGAACCGGAATTGC-3′ | Sf29 deletion from SfMNPV bacmid; forward primer with 25-nt homolog to Tn5-neo sequence (underlined) and 25-nt homolog to 3′ untranslated Sf29 region |
| Sf29del.2 | 5′-GTATTCATAACGCACCAACCCGGGTCAGAAGAACTCGTCAAGAAGGCG-3′ | Sf29 deletion from SfMNPV bacmid; reverse primer with 25-nt homolog to Tn5-neo sequence (underlined) and 25-nt homolog to 5′ untranslated Sf29 region |
| Sf29del.3 | 5′-AACTTTTAGGAAAATTATACACAATCAA TGATTTATTTATATTTTATTTACCC-3′ | Sf29 deletion from SfMNPV bacmid; forward primer with 25-nt homolog to Sf29del.1 (underlined) and 25-nt homolog to 3′ untranslated Sf29 region |
| Sf29del.4 | 5′-ATAAAATACTGCATCGTCGATGATTTTTATGTATTCATAACGCACCAACCC-3′ | Sf29 deletion from SfMNPV bacmid; reverse primer with 25-nt homolog to Sf29del.2 (underlined) and 25-nt homolog to 5′ untranslated Sf29 region |
| Sf29reg.1 | 5′-ATCTCAGACCATCTTGCCAA-3′ | Sf29 insertion into Sf29null bacmid; forward primer that amplifies 250 bp upstream of the Sf29 stop codon |
| Sf29reg.2 | 5′-GTATAGCGTCGACGACATCG-3′ | Sf29 insertion into Sf29null bacmid; reverse primer that amplifies 250 bp downstream of the Sf29 start codon |
| Sf29.1 | 5′-CTACTCAACGCGTTCAACGA-3′ | Sf29 transcription analysis (RT-PCR); forward primer that amplifies 250 bp downstream of the Sf29 stop codon |
| Sf29.2 | 5′-TTAGTCAAATAACGGCAATG-3′ | Sf29 transcription analysis (RT-PCR); reverse primer that amplifies in the Sf29 stop codon |
| Sfpolh.1 | 5′-CCCGACACCATGAAGCTGGT-3′ | Sfpolh transcription analysis (RT-PCR); forward primer that amplifies 500 bp upstream of the Sfpolh stop codon |
| Sfpolh.2 | 5′-TTAGTACGCGGGTCCGTTGTA-3′ | Sfpolh transcription analysis (RT-PCR); reverse primer that amplifies in the Sfpolh stop codon |