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Strategy for insertion of parental and mutant HaF gene constructs into the polyhedrin locus of the f-null HearNPV bacmid. All inserted genes were under the control of the Op166 promoter. All cassettes were inserted into the attb site (indicated by right and left insertion sites, Tn7R and Tn7L) in the polyhedrin locus by transposon-based transposition. The names of recombinant bacmids are listed in the box. egfp, enhanced green fluorescent protein.
