FIG. 2.

γHV68 induces proteasome-dependent degradation of PML. (A) Immunoblot of infected 3T12 cell extracts harvested at the indicated time points postinfection. The blots were probed with antibodies to various cellular proteins, indicated on the right-hand end of the panels. Molecular weights are indicated to the left of each panel. (B) RNA extracted from γHV68-infected cells was analyzed by quantitative RT-PCR (qRT-PCR). mRNA levels for PML and γHV68 ORF57 were normalized to β-actin. The data are representative of three independent experiments. The panel on the right shows a small sample of the infected cells used to obtain RNA, which was analyzed by immunoblotting for PML as a control. (C) Immunoblot of mock-infected and γHV68-infected 3T12 cells treated with either nothing (−), solvent (dimethyl sulfoxide [DMSO]), MG132, or lactocystin. The cell extracts were probed for expression levels of the proteins indicated to the left of each panel.