FIG. 1.
Analysis of the expression of both GFP and HIV-1 products in CEMGFP cells upon HIV-1 infection or cocultivation with U937HIV-1 cells. (A) FACS analyses for the detection of both GFP and HIV-1 Gag products in CEMGFP cells at different times after infection with the NL4-3 HIV-1 strain. The percentages of cells positive for either fluorescence are indicated at the late time points. (B) FACS analyses for the detection of both GFP and HIV-1 Gag products in U937HIV-1/CEMGFP cocultures carried out for 4, 8, and 16 h. In the top row, the analyses of the single-cell populations are shown, together with the percentages of HIV-1 Gag-positive U937HIV-1 cells. In the bottom row, the coculture analyses, including the percentages of events scored in the CEMGFP-related quadrants, are shown. (C) FACS analyses for the presence of GFP and HIV-1 Gag (left) or Env gp120 (right) in CEMGFP cells cocultured for 16 h with U937HIV-1 cells. The percentages of events scored in the CEMGFP-related quadrants are reported. The results are representative of two (A), four (B), and two (C) independent experiments.