Skip to main content
. Author manuscript; available in PMC: 2008 Aug 25.
Published in final edited form as: Mol Plant. 2008;1(2):338–346. doi: 10.1093/mp/ssn001

Figure 3. BL Induces Rapid BIN2 Depletion in a Dosage-Dependent Manner.

Figure 3

The gBIN2:GFP or gbin2-1:GFP transgenic seedlings were germinated and grown on BRZ-containing medium for 14 d and transferred to liquid medium containing various concentrations of BL. The seedlings were removed at different time points for the IP/Western analysis of BIN2:GFP (upper strip). The relative amounts of total protein extracts of each sample were analyzed by Western blot using the anti-BRI1 antibody (lower strip).

(A) The time course of BL-induced BIN2 depletion.

(B) The dosage-dependency of the BL-induced BIN2 disappearance.

The time-course (C) and dosage-dependency (D) tests of the effect of BL on the accumulation of bin2-1:GFP.