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. Author manuscript; available in PMC: 2008 Aug 25.
Published in final edited form as: Mol Plant. 2008;1(2):338–346. doi: 10.1093/mp/ssn001

Figure 7. The BL-Induced BIN2 Degradation is an Important Regulatory Mechanism for BIN2 Regulation.

Figure 7

gBIN2:GFP transgenic seedlings were grown on BRZ-containing medium for 2 weeks and transferred to liquid medium containing 100 mM LiCl, 1 μM BL or 1 μM BL plus 10 μM MG132. After immunoprecipitation, BIN2:GFP was used as the kinase for an in vitro phosphorylation assay with GST:BZR1. The amount of immunoprecipitated BIN2:GFP proteins was examined by Western blotting with the monoclonal GFP antibody (top panel). Longer exposure of the blot revealed a ladder of weak bands (indicated by three arrows in the second panel). The phosphorylation levels of GST:BZR1 were visualized by autoradiography (the third panel), and the amounts of the substrate used in the assay were shown by Coomassie Blue staining (bottom panel).