FIG. 7.

Importance of the TCV H5 small symmetrical loop. (A) Boxed nucleotides denote residues comprising the small symmetrical loop in TCV H5. Mutations generated in the loop are indicated. Triangles denote deleted bases. (B) Two mutants (H5/C-Δ and H5/G-Δ) assayed in planta produced progeny with single second-site alterations. Second-site changes within H5 and the number of clones containing the alterations at 20 dpi out of 16 clones sequenced are shown. Possible H5 structures of H5/C-Δ containing an adenylate-to-guanylate alteration at position 4002 (H5/C-ΔA4002G) and H5/G-Δ containing a cytidylate-to-adenylate alteration at position 4003 (H5/G-ΔC4003A) are shown. The second-site mutations are circled. (C) Relative accumulation levels of mutants in comparison to TCV-TSNLS (TCV) in protoplasts at 40 hpi. Results are the average of three experiments. Standard deviation bars are shown.