FIG. 2.

Infection of HOS and HeLa cells following coculture with MT2 cells. (A) HOS and HeLa reporter cells were cocultured with MT2 cells (MT2+HOS and MT2+HeLa) as described in Materials and Methods at a cell-to-cell ratio of 1:1 for 48 h with or without AZT (1 μM). Strongly EGFP-positive clusters in the AZT-treated column are likely due to syncytium formation. (B) Immunoblots of HTLV-1 gp46 (SU), p24 (CA), Tax, and actin. Coculture with MT2 cells (+) was carried out as described above for panel A. After coculture overnight, MT2 cells were removed by extensive washing. Two days after coculture, lysates of infected cells were prepared for immunoblotting with gp46, p24, Tax, and actin antibodies, respectively. Cell lysates from cocultures treated with 1 μM AZT (+) or not treated with AZT (−) are compared side by side.