TABLE 1.
Nucleotide and amino acid differences between the VB strain and HeLa-H-EAVP80 virus
| Protein(s) and region(s) (length in amino acids) | Genome region or ORF (nucleotide position)a | Nucleotide ina:
|
Amino acid inb:
|
||||
|---|---|---|---|---|---|---|---|
| Position | VB strain | HeLa-H- EAVP80 | Position | VB strain | HeLa-H- EAVP80 | ||
| None | 5′ UTR (1-224) | −c | − | − | |||
| Nonstructural (nsp) proteins | ORF 1ab (225-9751) | ||||||
| pp1ab polyprotein (3,175) | |||||||
| nsp1, Met1-Gly260 (260) | 658 | C | T | 145 | Ala | Val | |
| 695 | C | T | |||||
| nsp2, Gly261-Gly831 (571) | 1280 | C | T | ||||
| 1889 | T | C | |||||
| 1954 | A | G | 577 | Asp | Gly | ||
| 1979 | C | T | |||||
| 2057 | C | T | |||||
| 2483 | A | T | |||||
| nsp3, Gly832-Glu1064 (233) | 2898 | C | T | ||||
| 3011 | G | A | |||||
| nsp4, Gly1065-Glu1268 (204) | 3707 | Yd | C | ||||
| 3797 | T | C | |||||
| nsp5, Ser1269-Glu1430 (162) | 4349 | G | A | ||||
| nsp6, Gly1431-Glu1452 (22) | − | − | − | − | − | − | |
| nsp7, Ser1453-Glu1677 (225) | 4900 | A | G | 1559 | Lys | Arg | |
| nsp8, Gly1678-Asn1727 (50) | — | — | — | — | — | — | |
| nsp9, Gly1678-Glu2370 (693) | 6020 | C | T | 1933 | Pro | Ser | |
| 6752 | T | C | |||||
| 7021 | Rd | A | |||||
| nsp10, Ser2371-Gln2837 (467) | 8260 | Y | C | ||||
| 8290 | Y | C | |||||
| 8398 | T | C | |||||
| nsp11, Ser2838-Glu3056 (219) | − | − | − | − | − | − | |
| nsp12, Gly3057-Val3175 (119) | − | − | − | − | − | − | |
| Structural proteins | |||||||
| E (67) | ORF 2a (9751-9954) | 9867 | T | C | |||
| 9907 | A | T | 53 | Ser | Cys | ||
| 9914 | T | C | 55 | Val | Ala | ||
| GP2 (227) | ORF 2b (9824-10507) | 9867 | T | C | 15 | Leu | Ser |
| 9907 | A | T | |||||
| 9914 | T | C | 31 | Trp | Arg | ||
| 10082 | G | C | 87 | Val | Leu | ||
| 10157 | G | A | 112 | Ala | Thr | ||
| 10189 | T | C | |||||
| GP3 (163) | ORF 3 (10306-10797) | 10648 | A | G | 115 | Ser | Gly |
| 10709 | T | C | 135 | Leu | Pro | ||
| GP4 (152) | ORF 4 (10700-11158) | 10709 | T | C | 4 | Tyr | His |
| 11024 | A | T | 109 | Ile | Phe | ||
| GP5 (255) | ORF 5 (11146-11913) | 11171 | T | C | 9 | Phe | Ser |
| 11438 | C | T | 98 | Pro | Leu | ||
| 11475 | T | C | |||||
| 11704 | T | C | |||||
| M (162) | ORF 6 (11901-12389) | − | − | − | − | − | − |
| N (110) | ORF 7 (12313-12645) | − | − | − | − | − | − |
| None | 3′ UTR (12646-12704) | − | − | − | |||
a
Nucleotides are numbered according to the published sequence of EAV030 virus (GenBank accession no. NC_002532).
b
Only nonsynonymous mutations are shown, and silent mutations are not shown. Amino acids of nonstructural proteins are numbered according to their locations in the replicase polyprotein pp1ab. Amino acids of structural proteins are numbered according to their locations in the individual structural proteins.
c
−, no nucleotide or amino acid change occurred.
d
Y, T or C; R, A or G.