FIG. 5.

D248F mutation prevents V-mediated STAT2 cytoplasmic sequestration. 2fTGH cells were transiently transfected with empty vector or expression plasmids for the WT or mutant measles virus V (MeV) proteins indicated. Cells were left unstimulated (UNT) or were treated with 1,000 U of IFN-α/ml for 30 min prior to fixation, permeabilization, and sequential staining for a FLAG epitope tag to detect V protein and antiserum to detect endogenous STAT2. Arrows point to V-expressing cells.