FIG. 1.

The maintenance of KSHV-mediated lymphatic reprogramming requires KSHV gene expression. TIME cells were mock or KSHV infected and passaged at 2, 6, 10, 14, and 20 days postinfection (dpi). At 21 dpi, cells were again either KSHV or mock infected. Cell lysates were harvested on the indicated dpi and then subjected to immunoblot analysis with the indicated antibodies. Immunofluorescence for the latent KSHV LANA gene (% infected) was performed to monitor KSHV episome loss. VEGFR-3 and podoplanin expression correlates with KSHV episome loss. ND, not determined.