9AA-induced cell death in HTLV-1-transformed cells is concentration and time dependent. For the analysis of cell viability, a CellTiter-Glo luminescent cell viability kit (Promega) was used. (A) C8166 (C81), Hut102, and MT2 cells were treated with 5 to 20 μM 9AA for 48 h. At the end of the incubation period, cell viability was determined. (B) C8166, Hut102, and MT2 cells were treated with 20 μM 9AA for 24 or 48 h. At the end of the incubation period, cell viability was determined.