TABLE 2.
Activity assay of HCoV-HKU1
| Substratea | Km (μM) | kcat |
|---|---|---|
| C | 83.2 ± 13.3 | 1.1 ± 0.12 |
| H | 22.3 ± 5.2 | 0.09 ± 0.013 |
| SMC | 265.3 ± 21.5 | 0.03 ± 0.001 |
a
Substrate C refers to the consensus substrate of CoV Mpros with the sequence MCA-AVLQSGFR-Lys(Dnp)-Lys-NH2. Substrate H refers to the specific substrate of HCoV-HKU1, including a mutation at the P1 site from glutamine to histidine, with the sequence MCA-PRLHCTTN-Lys(Dnp)-Lys-NH2. Substrate SMC refers to the consensus substrate, including a mutation at the P1 site from glutamine to histidine, with the sequence MCA-AVLHSGFR-Lys(Dnp)-Lys-NH2.