Abstract
Acidic phospholipids neutralized the antistaphylococcal activity of rabbit, human, and rat serum and purified rabbit β-lysin as well. Several tissue bactericidal factors and cationic antibiotics were also neutralized. The neutralizing ability of all anionic lipids tested, together with the lack of this ability for the electrostatically neutral phosphatidylcholine, suggested that neutralization resulted from formation of electrostatic complexes between negative charges on the lipids and positive charges on the bactericidal substances. The neutralization of these host defense systems by phospholipids can explain the ability of phospholipids to increase bacterial virulence.
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Selected References
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- BLOMSTRAND R., NAKAYAMA F., NILSSON I. M. Identification of phospholipids in human thrombocytes and erythrocytes. J Lab Clin Med. 1962 May;59:771–778. [PubMed] [Google Scholar]
- DONALDSON D. M., ELLSWORTH B., MATHESON A. SEPARATION AND PURIFICATION OF BETA-LYSIN FROM NORMAL SERUM. J Immunol. 1964 Jun;92:896–901. [PubMed] [Google Scholar]
- FELIX K. Protamines. Adv Protein Chem. 1960;15:1–56. doi: 10.1016/s0065-3233(08)60307-1. [DOI] [PubMed] [Google Scholar]
- Gadebusch H. H., Johnson A. G. Natural host resistance to infection with Cryptococcus neoformans. V. The influence of cationic tissue proteins upon phagocytosis and on circulating antibody synthesis. J Infect Dis. 1966 Dec;116(5):566–572. doi: 10.1093/infdis/116.5.566. [DOI] [PubMed] [Google Scholar]
- HIGASHI Y., KURIMURA T., KUWAHARA O., OZAKI M., AMANO T. STUDIES ON THE ROLE OF PLAKIN. X. EFFECT ON MEMBRANE PHOSPHOLIPIDS. Biken J. 1963 Jul;6:111–126. [PubMed] [Google Scholar]
- HIRSCH J. G. Comparative bactericidal activities of blood serum and plasma serum. J Exp Med. 1960 Jul 1;112:15–22. doi: 10.1084/jem.112.1.15. [DOI] [PMC free article] [PubMed] [Google Scholar]
- KLEIN F., HAINES B. W., MAHLANDT B. G., DEARMON I. A., Jr, LINCOLN R. E. DUAL NATURE OF RESISTANCE MECHANISMS AS REVEALED BY STUDIES OF ANTHRAX SEPTICEMIA. J Bacteriol. 1963 May;85:1032–1038. doi: 10.1128/jb.85.5.1032-1038.1963. [DOI] [PMC free article] [PubMed] [Google Scholar]
- MUSCHEL L. H., TREFFERS H. P. Quantitative studies on the bactericidal actions of serum and complement. I. A rapid photometric growth assay for bactericidal activity. J Immunol. 1956 Jan;76(1):1–10. [PubMed] [Google Scholar]
- MYRVIK Q. N., SOTO-FIGUEROA E., NAFF G., HENELT E. Studies on antibacterial factors in mammalian tissues and fluids. II. Cofactor requirements of a serum bactericidin for Bacillus subtilis. J Immunol. 1958 Aug;81(2):118–122. [PubMed] [Google Scholar]
- NEWTON B. A. The properties and mode of action of the polymyxins. Bacteriol Rev. 1956 Mar;20(1):14–27. doi: 10.1128/br.20.1.14-27.1956. [DOI] [PMC free article] [PubMed] [Google Scholar]
- NUNGESTER W. J., AMES A. M. Effect of selected chemical substances on phagocytosis. J Infect Dis. 1952 Jan-Feb;90(1):51–60. doi: 10.1093/infdis/90.1.51. [DOI] [PubMed] [Google Scholar]
- RHODES D. N., LEA C. H. Phospholipids. IV. On the composition of hen's egg phospholipids. Biochem J. 1957 Mar;65(3):526–533. doi: 10.1042/bj0650526. [DOI] [PMC free article] [PubMed] [Google Scholar]
- SAWYER W. D., KUEHNE R. W., GOCHENOUR W. S., Jr EFFECT OF EGG YOLK AND PHOSPHATIDES ON ANTHRAX INFECTION OF RATS AND GUINEA PIGS. Proc Soc Exp Biol Med. 1965 Jan;118:105–108. doi: 10.3181/00379727-118-29769. [DOI] [PubMed] [Google Scholar]
- SKARNES R. C., WATSON D. W. Antimicrobial factors of normal tissues and fluids. Bacteriol Rev. 1957 Dec;21(4):273–294. doi: 10.1128/br.21.4.273-294.1957. [DOI] [PMC free article] [PubMed] [Google Scholar]