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. 2008 Jul 2;82(17):8687–8694. doi: 10.1128/JVI.00852-08

FIG. 1.

FIG. 1.

Construction and characterization of an L1-inducible virus. (A) Diagram of vL1Ri. The relevant segment of the viral genome is shown. Abbreviations: T7 Pol, bacteriophage T7 RNA polymerase open reading frame; lacO, E. coli lac operator; lacI, E. coli lac repressor open reading frame; P11, VACV late promoter; PE/L and P7.5, VACV early/late promoters; PT7, T7 promoter; EGFP, EGFP open reading frame. (B) Plaque formation by vL1Ri in the absence and presence of IPTG. BS-C-1 cells were infected with vL1Ri in the absence (−) or presence (+) of 50 μM IPTG. After 48 h, monolayers were fixed and stained with crystal violet. (C) Cells were infected as for panel B except that at 24 h the plate was examined with a fluorescence microscope for EGFP expression.