FIG. 3.

The replication defect of E4-deleted mutant Ad5 is rescued in the absence of functional MRN complex. (A) Immunoblot of lysates from NBS cells infected with the E4-deleted virus dl1004. Infections were compared in NBS cells transduced with an empty retrovirus vector or with a vector that expresses full-length Nbs1 protein. DBP demonstrated that early virus protein synthesis was comparable in the two cell lines. M represents lysates from mock infection, and Ku86 served as a loading control. (B) Replication of the E4-deleted virus is similar to wild-type Ad5 in NBS cells transduced with empty vector but is significantly compromised in cells expressing Nbs1. Cells were infected with either wild-type Ad5 or dl1004, and viral DNA extracted over a time course was analyzed by qPCR. Accumulation of viral DNA is represented as the fold increase over the input viral DNA, determined at the 4-h time point.