FIG. 6.

Nuclear localization of Mre11 is not sufficient to inhibit Ad replication. (A) Localization of Mre11 and Rad50 proteins in the absence and presence of virus infection. NBS cells overexpressing wild-type (WT) Mre11 and Mre11-NLS were infected with dl1004 mutant Ad and analyzed by immunofluorescence at 24 hpi. The Mre11-NLS protein was detected with an antibody to the HA epitope tag. Virus replication centers were visualized by staining with antibody to DBP, and cell nuclei were marked by staining with DAPI. (B) Replication of E4-deleted virus is inhibited by Nbs1 but not by Mre11-NLS. NBS cells transduced with empty vector (LXIN) or vectors expressing Nbs1, wild-type Mre11, and Mre11-NLS were infected with the E4-deleted mutant virus dl1004. Viral DNA was extracted over a time course of infection for analysis by qPCR. Accumulation of viral DNA is represented as fold increase over input viral DNA, determined at the 4-h time point.