FIG. 4.

Analysis of purified virions by silver staining and Western blotting. (A) Five micrograms of unpurified (Extracellular medium) or purified virions from HeLa cells were loaded onto a 12% SDS-polyacrylamide gel and silver stained. As controls, equivalent amounts of total mock-treated and infected cell lysates were also loaded. Note the differences between the protein pattern of purified virions and that of control total cell lysates (dots at right). (B) The same samples were also analyzed by Western blotting with the Remus antibody, but in this case, 25 μg of control mock and infected total cell lysates was loaded. Viral enrichment of the purified virions was particularly evident compared to that of the unpurified sample. As before, the molecular weights (MW) of the protein markers are indicated at the left of the gels (10³).