TABLE 1.

Viral content of extracellular virions

Protein group (expected in virions)a	Protein	MWb	Size (aa)c	No. of unique peptidesd	% Coveragee	% of totalf	Total no. of proteins
Capsid
UL6 (+)		74.1	676	1	2.5
UL15 (?)	Terminase	80.9	735	−	NA
UL17 (+)		74.6	703	10	23.8
UL18 (+)	VP23	34.3	318	15	65.7
UL19 (+)	VP5/ICP5	149.1	1,374	54	59.5
UL25 (+)		62.7	580	8	19.5
UL26 protease	VP24 (+)	26.6	247	7	41.3
	VP21 (−)	37.3	363	−	NA
	COOH termini (−)	2.6	25	−	NA
UL26.5	VP22a (−)	31.2	304	−	NA
	COOH termini (−)	2.6	25	−	NA
UL28 (?)	ICP18.5	85.6	785	−	NA
UL33 (?)		14.4	130	−	NA
UL35 (+)	VP26	12.1	112	3	39.3
UL38 (+)	VP19C	50.3	465	9	23.9
Total				107		26.7	8
Envelope
UL1 (+)	gL	24.9	224	4	24.6
UL10 (+)	gM	51.4	473	2	11.2
UL20 (+)		24.2	222	−	NA
UL22 (+)	gH	90.4	838	11	18.7
UL27 (+)	gB	100.3	904	22	30.2
UL34 (−)		29.8	275	−	NA
UL43 (?)		44.9	434	−	NA
UL44 (+)	gC	55.0	511	7	26.6
UL45 (+)		18.2	172	5	39.0
UL49A (+)	gN	9.2	91	−	NA
UL53 (?)	gK	37.6	338	−	NA
UL56 (+)		21.2	197	2	22.8
US4 (+)	gG	25.2	238	2	3.8
US5 (?)	gJ	9.6	92	−	NA
US6 (+)	gD	43.3	394	7	23.2
US7 (+)	gI	41.4	390	4	16.7
US8 (+)	gE	59.1	550	9	18.4
US8A (?)		16.8	159		NA
US9 (+)		10.0	90	1	37.8
Total				76		19	12g
Tegument
RL1 (+)	ICP34.5	26.2	248	1	3.4
RL2 (?)	ICP0	78.5	776	10	21.3
RS1 (?)	ICP4	132.8	1,298	20	22.6
UL4 (−)		78.2	718	−	NA
UL7 (?)		33.1	296	4	25.0
UL11 (+)		10.5	96	2	20.8
UL13 (+)		57.2	518	3	10.0
UL14 (+)		23.9	219	1	10.5
UL16 (+)		40.4	373	7	32.4
UL21 (+)		57.6	535	9	27.5
UL23 (?)	TK	41.0	376	1	4.4
UL31 (−)		34.0	306	−	NA
UL36 (+)	Large tegument	335.9	3,164	42	20.7
UL37 (+)	ICP32	120.6	1,123	22	33.2
UL41 (+)	Vhs	54.9	489	9	30.1
UL46 (+)	VP11/12	78.2	718	16	34.3
UL47 (+)	VP13/14	73.8	693	25	51.2
UL48 (+)	VP16/ICP25	54.3	490	15	45.5
UL49 (+)	VP22	32.3	301	8	39.5
UL50 (?)	dUTPase	39.1	371	6	26.7
UL51 (+)		25.5	244	4	27.0
UL55 (?)		20.5	186	4	37.6
US2 (+)		32.5	291	5	15.6
US3 (+)		52.8	481	1	6.7
US10 (+)		34.1	312	3	13.7
US11 (+)		17.8	161	−	NA
Total				218		54.4	23
Other
UL2 (−)		36.3	334	−	NA
UL3 (−)		25.6	235	−	NA
UL5 (−)		98.7	882	−	NA
UL8 (−)		79.9	750	−	NA
UL9 (−)		94.3	851	−	NA
UL12 (−)		67.5	626	−	NA
UL24 (?)		29.5	269	−	NA
UL29 (−)	ICP8	128.4	1,196	−	NA
UL30 (−)		136.4	1,235	−	NA
UL32 (−)		64.0	596	−	NA
UL39 (−)	ICP6/10	124.1	1,137	−	NA
UL40 (−)		38.0	340	−	NA
UL42 (−)		51.2	488	−	NA
UL52 (−)		114.4	1,058	−	NA
UL54 (−)	ICP27	55.3	512	−	NA
US1 (−)	ICP22	46.5	420	−	NA
US12 (−)	ICP47	9.8	88	−	NA

^aLocalization is based on that described in references 2 and 56. +, expected; −, not expected; ?, uncertain.

^bMW, estimated molecular weight (10³).

^caa, amino acids.

^d−, no peptides found.

^eValues shown are the percentages of coverage of proteins by peptides. NA, not applicable.

^fRelative peptide abundance.

^gThirteen proteins if UL20 is included (Table 2, MRM data).