TABLE 3.
Potential cellular proteins associated with extracellular virions identified by mass spectrometry
| Protein name | MWa | Size (aa)b | Number of unique peptides | % Coveragec |
|---|---|---|---|---|
| 14-3-3 protein | 28.2h | 248 | 1d | 4.0 |
| 14-3-3 protein epsilon | 29.2 | 255 | 3 | 16.9 |
| 14-3-3 protein gamma | 28.3 | 247 | 1 | 5.7 |
| 14-3-3 protein zeta/delta | 27.7 | 245 | 2 | 12.7 |
| Actin | 41.8h | 375 | 3e | 16.3 |
| Annexin A1 | 38.7 | 346 | 1 | 4.6 |
| Annexin A2 | 38.6 | 339 | 3 | 13.6 |
| Annexin A5 | 35.9 | 320 | 1 | 5.0 |
| Arf1/Arf 3 | 20.7 | 181 | 3f | 26.0 |
| Arf1/Arf3/Arf5 | 20.6 | 181 | 2f | 11.0 |
| Arf1/Arf3/Arf4/Arf5 | 20.6 | 181 | 2f | 16.0 |
| Arf4 | 20.5 | 180 | 1 | 10.0 |
| ATP-dependent RNA helicase DDX3X | 73.2 | 662 | 2 | 3.3 |
| Casein kinase 2 | 45.1 | 391 | 1 | 4.6 |
| Cofilin 1 | 18.5 | 166 | 1 | 7.4 |
| Cyclophilin A | 18.0 | 164 | 8 | 64.8 |
| Cystein-glycin-rich protein 1 | 20.6 | 193 | 1 | 11.4 |
| Eukaryotic translation initiation factor 4H (eIF4H) | 27.4 | 248 | 4 | 28.2 |
| GAPDHi | 36.1 | 335 | 1 | 4.2 |
| Growth factor receptor bound protein 2 | 25.2 | 217 | 1 | 6.5 |
| HSP70 | 70.4 | 641 | 3 | 7.8 |
| Keratin 1 | 66.0 | 644 | 1 | 2.5 |
| Keratin 10 | 59.5 | 593 | 1 | 2.0 |
| Macrophage migration inhibitory factor | 12.5 | 115 | 1 | 9.5 |
| Membrane attack complex inhibition factor (CD59) | 14.2 | 128 | 1 | 9.4 |
| Nucleoside diphosphate kinase A/B | 17.3 | 152 | 1 | 11.8 |
| Peroxiredoxin-1 | 22.1 | 199 | 2 | 10.6 |
| Peroxiredoxin-2 | 21.9 | 198 | 1 | 5.5 |
| Profilin-1 | 15.1 | 140 | 4 | 28.6 |
| Programmed cell death protein 6 | 21.9 | 191 | 2 | 10.5 |
| Rab | 24.0h | 215 | 3g | 15.3 |
| Rab2A/Rab 2B/Rab4B | 23.8 | 214 | 1 | 6.1 |
| Rab5 | 23.6 | 215 | 1 | 5.1 |
| Rab6 | 25.2 | 223 | 3 | 21.1 |
| Rab7A | 23.5 | 207 | 1 | 6.8 |
| Rab10 | 22.5 | 200 | 1 | 5.5 |
| Rab11 | 24.5 | 217 | 2 | 11.1 |
| Rab15 | 24.4 | 212 | 1 | 5.2 |
| Rab33B | 25.7 | 229 | 1 | 4.8 |
| Rab35 | 23.0 | 201 | 1 | 5.5 |
| Rab-like protein 3 | 26.4 | 236 | 1 | 4.7 |
| S100 calcium protein binding A11 | 11.7 | 105 | 2 | 25.7 |
| Sec14-like protein 4 | 46.6 | 406 | 1 | 2.2 |
| Tetraspanin 13 | 22.2 | 204 | 1 | 10.3 |
| Transferrin receptor protein 1 (CD71) | 84.9 | 760 | 1 | 1.2 |
| Translocase of inner mitochondrial membrane 50 (TIMM50) | 39.6 | 353 | 1 | 3.1 |
| Triosephosphatase isomerase | 26.7 | 249 | 1 | 11.7 |
| Ubiquitin C | 8.6 | 76 | 2 | 32.9 |
| Ubiquitin-conjugating enzyme E2 L3 | 17.9 | 154 | 1 | 14.3 |
| Total peptides | 85 |
a
MW, estimated molecular weight (103).
b
aa, amino acids.
c
Percentage of coverage of protein by polypeptides detected by tandem mass spectrometry.
d
Shared among the 14-3-3 protein family members.
e
Shared among the actin family members.
f
Shared among the indicated Arf proteins.
g
Shared among the Rab protein family members.
h
MW was averaged for these family members.
i
GAPDH, glyceraldehyde-3-phosphate dehydrogenase.