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. 2008 Jul 2;82(17):8373–8382. doi: 10.1128/JVI.00670-08

FIG. 5.

FIG. 5.

Genetic dissociation of Nef-induced CD4 downregulation and Ii chain upregulation. (A) Twenty-seven pediatric nef alleles were tested for their abilities to downregulate CD4 in Jurkat cells and to upregulate the Ii chain in HeLa-CIITA cells. Data obtained from at least three independent experiments were normalized. Each dot represents one of the 27 nef alleles. (B) Vesicular stomatitis virus G protein-pseudotyped HIV-1 NL4-3 IRES-eGFP viruses containing either the NL4-3 wild-type nef gene or its two mutant forms A83L and LIH100IYY were tested for CD4 and MHC-I downmodulation on phytohemagglutinin-activated PBMC and Ii chain upregulation on THP-1 cells. Results from at least three independent experiments were normalized by taking the effects observed with Nef NL4-3 as 100%.