FIG. 2.
RT-PCR analysis of 5HT2aR expression in SVG-A cells and in cells differentiated to oligodendrocyte progenitors (OPCs). RNA was extracted from SVG-A cells and from the H7-derived progenitors. The RNA was reverse transcribed and cDNA amplified with 5HT2aR-specific primers. GAPDH was used as an internal control. 5HT2aR-specific products (141 bp) were amplified from both the SVG-A- and H7-derived progenitor populations only when reverse transcriptase was included in the reaction. The GAPDH bands are 96 bp, making them easily distinguishable on the gel.