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Maturation of α-factor precursor is incomplete in S(all)A Clc1p cells treated with pheromone. GPY1946 (WT CLC1, lanes 1 and 3) and GPY1949 [S(all)A clc1; lanes 2 and 4] were grown at 30°C, incubated without (lanes 1 and 2) or with (lanes 3 and 4) a-factor for 1 h, and metabolically labeled with [35S]cysteine/methionine for 30 min. α-Factor was immunoprecipitated from the culture supernatant and subjected to SDS-PAGE and phosphoimaging.
