Figure 11.

Kex2p stability is decreased in the S(all)A Clc1p mutant. Congenic strains containing an integrated copy of wild-type (WT) CLC1 (GPY1946) or S(all)A clc1 (GPY1949) were grown at 30°C, incubated without (A) or with (B) a-factor, and metabolically labeled with [³⁵S]cysteine/methionine for 15 min. Excess unlabeled amino acids were added, and an aliquot of cells was harvested from each sample at 0-, 45-, or 90-min intervals (chase). Cells were lysed, and Kex2p was immunoprecipitated and subjected to SDS-PAGE and phosphoimaging. Numbers below each lane correspond to the relative percentage of Kex2p remaining, normalized to the 0-min chase value.