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Casein kinase II is not responsible for Clc1p phosphorylation. Clc1p phosphorylation in a cka1Δ cka2Δ strain carrying either a wild-type (GPY987, WT) or temperature-sensitive (GPY988, cka2-ts) copy of CKA2. Wild-type cells were grown at 24°C and then incubated at the permissive temperature (24°C) for 90 min or the nonpermissive temperature (37°C) for 60 min. Cells were then treated with or without 2.5 μM α-factor for 30 min and labeled with [32P]Pi for 30 min.
