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. 1999 Mar;10(3):713–726. doi: 10.1091/mbc.10.3.713

Figure 7.

Figure 7

Steady-state levels of Chc1p in Clc1p mutants are not significantly altered. Protein extracts were prepared from GPY74-15C (which carries endogenous CLC1, lane 1) or GPY1034-19D (clc1Δ cells containing pgalCLCURA3, lane 2), GPY1970 (wild-type [WT] CLC1 integrated at the LEU2 locus, lane 3), and GPY1973 [S(all)A clc1 integrated at the LEU2 locus, lane 4]. Growth of GPY1034-19D cells in 2% glucose repressed Clc1p expression. Extracts were subjected to SDS-PAGE, transferred to nitrocellulose, and probed with either monoclonal antibodies to Chc1p or polyclonal antibodies to Clc1p. The lower band corresponds to a Chc1p degradation product.