FIG. 6.

Cox1 is translated in the absence of COA2. (A) COA2 was deleted in arg8Δ strains in which ARG8 replaces the COX1 codons (cox1Δ::ARG8) or ARG8 is fused to 512 codons of COX1 [COX1(1-512)::ARG8]. The strains were grown in SC-2% glucose containing 0.2× Arg (4 mg/liter), serially diluted, and spotted on YPD, SC-2% glucose with or without 1× Arg, and YPLG. The plates were incubated at 30°C for 2 days (glucose plates) or 4 days (YPLG). The arg4Δ strain is shown as a control. (B) In vivo labeling of mitochondrial translation products of the cox1Δ::ARG8 strain, with or without the COA2 gene. The cells were treated as described in the legend to Fig. 5A. The additional band is the mature Arg8 protein. WT corresponds to the W303 strain labeled for 20 min. (C) The COX1(1-512)::ARG8 strain, with or without the COA2 gene, was radiolabeled as described in the legend to Fig. 5A. The bands corresponding to the Cox1::Arg8 fusion and to the mature Arg8 protein are marked. WT corresponds to the W303 strain labeled for 20 min. (D) coa2Δ yta12Δ cells transformed with a centromeric vector expressing Yta12(E614Q) were grown in SC-2% raffinose, serially diluted, and spotted on YPLG or YPD. The plates were incubated at 30°C for 2 days (YPD) or 6 days (YPLG).