FIG. 7.

Interaction of hERG1 isoforms in tumor cells. Coprecipitation of USO isoforms with hERG1A and/or hERG1B in SH-SY5Y cells (A) and FLG 29.1 cells (B). Proteins (2 mg) were immunoprecipitated with anti-USO antibody (IP anti-USO) and run through a 7.5% SDS-polyacrylamide gel, and the following WB was revealed with anti-pan-hERG1 antibody. Total lysates from each cell line and from HEK-hERG1A and -hERG1B-expressing cells (HEK-hERG1A-B) were also loaded. The results of WB analysis of protein extracts (50 μg/lane) from SH-SY5Y (A) and FLG 29.1 (B) cells probed with anti-USO antibody are shown in the rightmost gels (WB: anti-USO). The positions of molecular mass standards (in kilodaltons) are indicated to the left of the gels. The no-IP gels contain samples to which no antibody was added to cell lysates during immunoprecipitation.