FIG. 9.

STAP-2 displaces TRADD from LMP1. (a and b) 293T cells (1 × 10⁷ cells/well) were transfected with Myc-tagged STAP-2 (5 μg) with or without HA-tagged TRADD or FLAG-tagged RIP1 (10 μg). At 48 h after transfection, the cells were lysed, immunoprecipitated (IP) with an anti-HA (a) or anti-FLAG (b) antibody and immunoblotted (IB) with an anti-Myc or an anti-FLAG antibody. An aliquot of each total cell lysate (TCL) was immunoblotted with the anti-Myc antibody. (c) 293T cells (1 × 10⁷) were transfected with FLAG-tagged LMP1 (10 μg) or HA-tagged TRADD (10 μg) and/or Myc-tagged STAP-2 (5 μg). At 48 h after transfection, the cells were lysed, immunoprecipitated with anti-FLAG antibody, and immunoblotted with an anti-HA, an anti-Myc, or an anti-FLAG antibody. An aliquot of each TCL was immunoblotted with the anti-HA or anti-Myc antibody. (d) 293T cells (1 × 10⁷ cells/well) were transfected with HA-tagged LMP1 (10 μg) or FLAG-tagged RIP1 (10 μg) and/or Myc-tagged STAP-2 (5 μg). At 48 h after transfection, the cells were lysed, immunoprecipitated with anti-HA antibody and immunoblotted with an anti-FLAG, an anti-Myc, or an anti-HA antibody. An aliquot of each TCL was immunoblotted with the anti-FLAG or anti-Myc antibody.