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Quantitation of γH2AX foci in WT and ERCC1-XPF-deficient cells exposed to IR. Histograms indicate the fractions of cells with no foci (blue), one or two foci (green), or more than two foci (red) at 0, 4, 12, 24, and 48 h postirradiation. Panels: A, immortalized WT human fibroblasts (C5RO); B, immortalized XPF-deficient human fibroblasts (XFE); C, WT primary MEFs; D, Ercc1−/− primary MEFs; E, WT ES cells; F, Ercc1−/− ES cells.
