FIG. 1.

Effects of mutations in septin nucleotide-binding motifs on cell viability. (A) Schematic diagram of a typical septin. Filled boxes show the approximate locations of septin P-loop, G3, and G4 domains and of the C-terminal predicted coiled-coil region. Indicated are the amino acid sequences of the septin P-loop, G3, and G4 motifs. A bold font indicates residues mutated singly or in combination in this study. (B to F) Viability of wild-type and mutant strains. Strains were grown to mid-log phase in SDC-Leu medium (B to D and F) or YM-P medium (E), and fivefold serial dilutions were plated on the appropriate plates followed by incubation at the indicated temperature for 4 days.