TABLE 2.

Septin localization during log-phase growth and S-phase arrest

Strain	Relevant genotype	Temp (°C)	% Budded cells with septins at the necka
			At time (h) in log phaseb						After hydroxyurea arrest atc:
			0	1	2	3	5	8	0 min	90 min
M-2437	CDC11	23	99	99	99	96	96	95	ND	ND
M-2437	CDC11	37	99	94	99	99	90	91	99	100
M-2962	cdc12-6	37	91	1	0	0	0	0	96	4
M-2452	cdc11G32E	37	100	50	24	5	5	3	93	96
M-2440	cdc11G32V	37	99	77	29	23	12	5	91	94
M-2443	cdc11R35T	37	99	96	94	95	94	90	99	98
M-2446	cdc11R35E	37	99	95	82	38	29	14	96	95
M-2449	cdc11S36N	37	97	94	60	53	27	11	ND	ND
M-3277	cdc11D89A, G92A	37	96	93	92	93	90	89	ND	ND
M-3280	cdc11K172A, D174A	37	92	31	12	1	0	0	ND	ND
M-2922	CDC3	37	92	93	87	87	85	86	90	87
M-2923	cdc3G129	37	96	95	90	92	89	86	93	92
M-2927	cdc3G129V, K132E, T133N	37	85	43	15	2	0	0	89	88
M-2855	CDC10	37	99	97	92	93	89	90	ND	ND
M-2856	cdc10G42V	37	97	46	30	12	7	4	86	78
M-2857	cdc10K45T	37	98	33	14	2	0	1	ND	ND
M-2863	CDC12	37	97	94	97	87	88	86	90	82
M-2864	cdc12G44V	37	92	73	52	29	10	4	91	87
M-2865	cdc12K47T	37	91	89	87	82	81	80	ND	ND
M-2866	cdc12G44V, K47T, T48N	37	79	27	10	0	0	0	ND	ND
M-2867	cdc12D98A, G101A	37	97	96	86	87	74	77	ND	ND

^aSeptin localization was determined by epifluorescence microscopy. More than 200 budded cells were counted per sample.

^bThe indicated strains were grown to mid-log phase at 23°C, and septin localization was determined before (0 h) and at the times indicated after shift to 37°C.

^cStrains were grown to mid-log phase at 23°C and arrested with 0.2 M hydroxyurea, and septin localization was determined before and after 90 min at 37°C. ND, not determined.