TABLE 4.

Two-hybrid interactions among S. cerevisiae wild-type and mutant septins

DBD fusion	β-Galactosidase activity with DBD fusiona
	pJG4-5	CDC3	CDC3-N	CDC3-C	CDC10	CDC11	CDC11-N	CDC11-C	cdc11G32V	cdc11R35T	cdc11R35E	cdc11S36N	cdc113nt	CDC12	CDC12-N	CDC12-C
Noneb	12	12	3	4	10	6	17	14	15	10	13	8	31	12	10	5
CDC3	15	28	2	21	26	2,803	150	7	10	931	8	8	5	1,942	618	8
cdc3G129V	8	11	6	19	16	361	112	7	5	101	13	5	6	635	235	15
cdc3K132T	19	23	23	18	43	869	178	27	19	366	41	31	41	1,084	311	43
cdc3T133N	6	18	37	35	13	663	148	4	14	215	32	15	15	1,160	205	23
CDC3-N	17	8	2	12	156	227	50	21	7	44	9	9	38	249	651	15
CDC3-C	14	20	5	4	27	54	82	3	15	52	40	41	6	1,853	29	201
CDC10	10	486	109	4	11	1,708	366	29	10	953	23	97	10	333	41	22
cdc10K45T	21	28	27	ND	ND	18	12	ND	21	16	38	ND	ND	30	40	ND
CDC11	19	11	2	3	40	2,192	60	5	9	1,421	7	10	9	1,381	1,843	6
CDC11-N	4	15	5	16	34	2,092	2,383	10	24	1,976	4	14	20	1,440	1,124	9
CDC11-C	3	14	14	14	7	13	51	28	30	4	1	9	5	15	15	10
cdc11G32V	5	2	2	14	10	14	10	14	5	2	2	4	3	16	15	13
cdc11R35T	8	15	10	16	38	2,104	52	8	9	836	10	35	7	1,052	717	7
cdc11R35E	7	15	5	11	7	37	5	10	12	6	8	8	8	695	719	8
cdc11S36N	4	14	6	11	15	13	3	6	6	7	6	11	10	1,129	994	7
cdc11 3nt	6	21	14	27	9	36	11	6	19	7	9	46	13	16	3	4
cdc11 G3	11	9	ND	ND	65	2,306	125	4	ND	ND	ND	ND	ND	2,021	2,357	5
cdc11 G4	10	23	ND	ND	11	13	8	16	ND	ND	ND	ND	ND	7	11	8
CDC12	7	157	5	63	15	2,568	954	11	10	1,820	654	1,622	10	2,784	77	7
CDC12-N	5	14	15	18	45	2,925	527	44	21	525	128	78	10	439	196	11
CDC12-C	7	211	10	389	26	16	40	7	6	5	2	48	8	1,538	77	138
cdc12G44V	6	121	ND	ND	14	852	311	5	ND	ND	ND	ND	ND	666	ND	ND
cdc12 3nt	4	14	ND	ND	7	12	7	10	ND	ND	ND	ND	ND	9	ND	ND
cdc12S43V	11	184	ND	ND	19	1,986	753	ND	ND	ND	ND	ND	ND	2,480	ND	ND

^aTwo-hybrid assays were performed as described in Materials and Methods. At least four independent β-galactosidase assays were done for each combination. Strains were grown at 23°C for 16 h in minimal medium containing leucine and 1% raffinose and 2% galactose. Shown are the average values in Miller units. Values above 100 and at least ninefold higher than the corresponding control (pEG202 with no insert) were deemed positive and are shown in boldface. -N and -C indicate the N- and C-terminal fragments, respectively, of the indicated gene (16). cdc11 G3 is cdc11^{D89A, G92A}, cdc11 G4 is cdc11^{K172A, D174A}, cdc11 3nt is cdc11^{G32V, R35E, S36N}, and cdc12 3nt is cdc12^{G44V, K47E, T48N}. ND, not done.

^bpEG202 with no insert.